Sequencing, identification and mapping of primed L1 elements (SIMPLE) reveals significant variation in full length L1 elements between individuals
نویسندگان
چکیده
منابع مشابه
A comprehensive analysis of recently integrated human Ta L1 elements.
The Ta (transcribed, subset a) subfamily of L1 LINEs (long interspersed elements) is characterized by a 3-bp ACA sequence in the 3' untranslated region and contains approximately 520 members in the human genome. Here, we have extracted 468 Ta L1Hs (L1 human specific) elements from the draft human genomic sequence and screened individual elements using polymerase-chain-reaction (PCR) assays to d...
متن کاملL1 Elements as a Source of Environmentally Sensitive Genetic Instability
LINE-1 is the only active autonomous non-LTR human retroelement. The expression and activity of these elements contribute to human genomic instability. LINE-1 elements comprise 17% of the human genome, which translates into about 5x10 L1 copies, the majority of which are defective due to truncations at their 5’ end. We have analyzed the expression profiles of endogenous L1 elements from differe...
متن کاملHigh-throughput sequencing reveals extensive variation in human-specific L1 content in individual human genomes.
Using high-throughput sequencing, we devised a technique to determine the insertion sites of virtually all members of the human-specific L1 retrotransposon family in any human genome. Using diagnostic nucleotides, we were able to locate the approximately 800 L1Hs copies corresponding specifically to the pre-Ta, Ta-0, and Ta-1 L1Hs subfamilies, with over 90% of sequenced reads corresponding to h...
متن کاملAn actively retrotransposing, novel subfamily of mouse L1 elements.
Retrotransposition of LINEs and other retroelements increases repetition in mammalian genomes and can cause deleterious mutations. Recent insertions of two full-length L1s, L1spa and L1Orl, caused the disease phenotypes of the spastic and Orleans reeler mice respectively. Here we show that these two recently retrotransposed L1s are nearly identical in sequence, have two open reading frames and ...
متن کاملcomprehension monitoring in l1&l2
هدف اصلی این تحقیق مقایسه و تمایز بین روشها و استراتژیهای خاص برای درک مطلب بهتر در زبان مادری و زبان دوم بوده است. در قدم اول میزان توانایی افراد در درک مطلب فارسی و انگلیسی اندازه گیری و سپس رده بندی شد. همچنین از دانش آموزان خواسته شد که در مورد روشها و استراتژیهایی که برای درک مطلب بهتر به کار برده بودند توضیح دهند. نتیجه این مرحله از تحقیق نشان داد که دانشجویان برای درک مطلب مطلوب در زبان ...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2015
ISSN: 1471-2164
DOI: 10.1186/s12864-015-1374-y